从各种高质量 96 孔板、模块和孔架以及相关配件中进行选择。
类别：DNAAllDNA 纯化质粒纯化清理/DNA 片段纯化自动/高通量 DNA 分析RNAAllRNA提取试剂盒RNA 清理试剂盒RNA 分析试剂盒DNA/RNA 共提取试剂盒DNA/RNA 共提取表观遗传学所有DNA/RNA 甲基化DNA 羟甲基化DNA 甲基化标准品NGS 文库制备试剂盒ChIP 试剂盒表观遗传学酶和试剂微生物组学所有微生物标准品样品收集DNA 分离RNA 分离DNA/RNA 共纯化分析样品收集所有DNA/RNA 屏蔽批量试剂拭子aliva粪便血液尿液环境/载体组织NGS 文库制备试剂盒AllRNA_Sequencing \">RNA 测序亚硫酸氢盐_测序\">亚硫酸氢盐测序微生物组_测序\">微生物组测序大肠杆菌所有感受态细胞培养基抗生素和化学品酵母所有DNA 分离RNA 分离蛋白质分离酵母转化酵母试剂酶和酶蛋白质和酶所有酶细菌表达菌株用于蛋白质表达的培养基标记蛋白质纯化柱和塑料所有试管裂解管柱96 孔板96 孔旋转板磁珠过滤器储液槽手动均质器设备和仪器所有真空歧管涡旋混合器均质器过滤器组件过滤器组件主体
类别：DNAAllDNA 纯化质粒纯化清理/DNA 片段纯化自动/高通量DNA 分析RNAAllRNA 提取试剂盒RNA 清理试剂盒RNA 分析试剂盒DNA/RNA 共提取试剂盒DNA/RNA 共提取表观遗传学所有DNA/RNA 甲基化DNA 羟甲基化DNA 甲基化标准NGS 文库制备试剂盒ChIP 试剂盒表观遗传酶和试剂微生物组学所有微生物标准品样品收集DNA 分离RNA 分离DNA/RNA 共纯化分析样品收集所有DNA/RNA Shield批量试剂拭子唾液粪便血液尿液环境/载体组织NGS 文库制备试剂盒所有RNA_测序\">RNA 测序亚硫酸氢盐测序\">亚硫酸氢盐测序微生物组_测序\">微生物组测序大肠杆菌所有感受态细胞培养基抗生素和化学品酵母所有DNA分离RNA分离蛋白质分离酵母转化酵母试剂和酶蛋白质和酶全部酶细菌表达菌株Culture M用于蛋白质表达的 edia 标记蛋白质纯化柱和塑料所有管裂解管柱96 孔板96 孔旋转板磁珠过滤器储液槽手动均质器设备和仪器所有真空歧管涡旋器均质器过滤器组件概述过滤器组件主体旋转柱正在寻找旋转柱？查看我们各种尺寸的带帽和不带帽旋转柱，适合您的核酸纯化研究。
类别：DNAAllDNA 纯化质粒纯化清理/DNA 片段纯化自动/高通量DNA 分析RNAAllRNA 提取试剂盒RNA 清理试剂盒RNA 分析试剂盒DNA/RNA共提取试剂盒DNA/RNA 共提取表观遗传学所有DNA/RNA 甲基化DNA 羟甲基化DNA 甲基化标准品NGS 文库制备试剂盒ChIP 试剂盒表观遗传酶和试剂微生物组学所有微生物标准品样品收集DNA 分离RNA 分离DNA/RNA 共纯化分析样品收集所有DNA/RNA 屏蔽批量试剂拭子唾液粪便血液尿液环境/载体TissueNGS 文库制备试剂盒AllRNA_Sequencing\">RNA 测序亚硫酸氢盐_Sequencing\">亚硫酸氢盐测序Microbiome_Sequencing \">微生物组测序大肠杆菌所有感受态细胞培养基抗生素和化学品酵母所有DNA分离RNA分离蛋白质分离酵母转化酵母试剂和酶蛋白质和酶所有酶细菌表达菌株蛋白质表达的培养基标记蛋白质纯化柱和塑料所有管裂解管柱96 孔板96 孔旋转板磁珠过滤器储液槽手动均质器设备和仪器所有真空歧管涡旋混合器均质器旋转柱概述种类繁多各种尺寸的加盖和未加盖旋转柱您的核酸纯化研究。After lysing the samples. DNA or RNA needs to be purified. If it is RNA sample the lysis buffer must contain something (2-merceptoethanol) that will destroy all RNAse enzyme activity.
Then you can purify samples in two ways,
a) Phenol-chloroform extraction,
b) Using kit column,
a) Phenol-chloroform extraction:
Materials:
Saturated phenol, chloroform, 3 M sodium acetate pH 5.2 or 5 M ammonium acetate, 100% ethanol or isopropanol.
Methods:
1. Add equal volume of saturated phenol:chloroform (1:1) to the DNA solution.
2. Mix well. Most DNA solutions can be vortexed for 10 sec. If the molecular weight of the DNA is high (eukaryotic genomic DNA) then sample should be gently rocked.
3. Spin in a microfuge for 3 min.
4. Carefully collect the supernatant layer to a new tube, being careful to avoid the interface. (repeat Steps 1-4 until an interface is no longer visible).
5. To remove traces of phenol, add an equal volume of chloroform to the aqueous layer.
6. Spin in a microfuge for 3 min.
7. Remove aqueous layer to new tube.
8. Measure the volume of the DNA sample. add 1/10 volume of sodium acetate, (final concentration of 0.3 M) or an equal volume of 5 M ammonium acetate (final concentration of 2.0-2.5 M). Mix well.
9. Add 2.5 volumes of cold 100% ethanol or isopropanol. Mix well.
10.Place on ice or at -20 degrees C for 20 minutes.
11.Spin a maximum speed in a microfuge 10-15 min. Carefully remove supernatant.
12.Add 1 ml 70% ethanol. Mix. Spin briefly. Carefully remove supernatant.
13.Air dry or briefly vacuum dry pellet.
14.Resuspend pellet in the appropriate volume of TE or water.
b) Using kit centrifuge column:
Materials:
Appropriate kit from Qiagen or Zymoresearch etc.
Methods:
Most of the company use the following common method to purify DNA.
1. Add 2 volumes of DNA binding solution. Mix it.
2. Transfer the mix to the column on a 2ml collection tube.
3. Spin at 10,000rpm for 10-30 sec. All the DNA will bind to the column.
4. Discard the solution in the collection tube and reuse the tube.
5. Add almost 200ul of washing solution containing 100% ethanol to the column.
6. Spin at 10,000rpm for 10-30 sec. Discard flow through column.
7. Repeat steps 5 and 6.
8. spin at 13,000rpm for 1 min to remove all ethanol residue.
9. Transfer the column to a 1ml eppendorf. add appropriate folume of TE or water directly on the column.
10.Spin for 2 min at high speed.
11.Store the DNA sample at -20 degree centrigade.
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