Description
| Applicable For | Next-Generation Sequencing, qPCR, microarray |
|---|---|
| Device Specs | 2 mL screwcap tube pre-filled with DNA/RNA Shield (1 mL) and ultra-high density BashingBeads (R1103 - 0.5 mm & 0.1 mm beads, R1105 - 2.0 mm beads) for homogenization. R1104 includes swab with 20 mm breakpoint. |
| Device Storage | Ambient temperature => 2 years |
| Sample Collection | 10% (v/v or w/v) |
| Sample Source | Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc. |
| Sample Stability | RNA: Ambient temperature (4°C-25°C) > 1 month DNA: Ambient temperature (4°C-25°C) > 2 years DNA & RNA: Frozen (< -20°C): Indefinitely |
Q1: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q2: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
Q3: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month DNA > 2 years
Q4: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°c):>
Q5: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
Q6: A white precipitate occurred after thawing frozen samples stored in DNA/RNA Shield, is this normal?
Some components of the reagent (or sample) may have precipitated out of solution during the freeze-thaw process. Once the sample is thawed to ambient temperature, vortex the sample to bring precipitate back into solution. Furthermore, try heating samples in hand or 37°C for 5 minutes and vortex.
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q8: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
-Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
-Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
