Highlights
- Simple: Quickly and easily rescue plasmid from yeast.
- Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
- High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description
| Applicable For | Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications |
|---|---|
| Elution Volume | ≥ 10 µl |
| Equipment | Microcentrifuge & Heat Block/Bath |
| Processing Volume | ≤ 1.5 ml of Culture |
| Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
| Size Range | Up to 23 kb. |
| Yield | Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures |
Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Q2: What is the typical plasmid yield?
Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures.In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.
Q3: Can this kit be used to isolate linear plasmid DNA?
Yes
Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Q5: What yeast strains are these kits compatible with?
Any strains susceptible to Zymolyase, which includes:Susceptible fungal genera:AsbyaKloekeraCandidaKluyveromycesDebaryomycesLipomycesEremotheciumMetschikowiaEndomycesPichiaHansenulaPullulariaHanseniasporaSaccharomycesSaccaromycodesSaccharomycopsisSchizosaccahromycesTorulopsis
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E1004 | Zymolyase | 1000 U | $75.00 | |
| D2004-1-10 | Solution 1 Digestion Buffer | 10 ml | $18.00 | |
| D2004-2-10 | Solution 2 Lysis Buffer | 10 ml | $18.00 | |
| D2004-3-20 | Solution 3 Neutralizing Buffer | 20 ml | $18.00 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| C1003-50 | Zymo-Spin I Columns | 50 Pack | $50.00 |
