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当前位置: 首页 > 产品中心 > Electrophoretic_buffer > ZYMO研究/Direct-zol-96 RNA试剂盒/2 x 96 preps/R2054
商品详细ZYMO研究/Direct-zol-96 RNA试剂盒/2 x 96 preps/R2054
ZYMO研究/Direct-zol-96 RNA试剂盒/2 x 96 preps/R2054
ZYMO研究/Direct-zol-96 RNA试剂盒/2 x 96 preps/R2054
商品编号: R2054
品牌: zymoresearch
市场价: ¥12560.00
美元价: 7536.00
产地: 美国(厂家直采)
公司:
产品分类: 电泳缓冲液
公司分类: Electrophoretic_buffer
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Isolate total RNA and small/miRNA from TRIzol without phase separation
Highlights

  • Easy Handling: Bypass chloroform, phase separation and precipitation steps.
  • NGS-Ready: Ultra-pure RNA without phenol carryover. No DNA contamination (DNase I included).
  • Non-Biased: Complete RNA recovery without miRNA loss.
Description

The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents.The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.The entire procedure typically takes about 30 minutes (per 2 plates).Learn More


CompatibilityTRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.
EquipmentMicroplate centrifuge, vortex
Sample InactivationTRI Reagent® (provided with R2055, R2057) inhibits RNase activity and inactivates viruses and other infectious agents.
Sample SourceAny sample stored and preserved in TRI Reagent®, TRIzol® or similar (animal cells, tissue, bacteria, yeast, fecal, biological fluids, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA)).
Size RangeTotal RNA ≥ 17 nt
Yield10 µg RNA (binding capacity), ≥10 µl (elution volume)

Q1: Is DNase I available for individual purchase?

All kit components are available for purchase separately.

Q2: How to store DNase-I following resuspension?

Lyophilized DNase I is stable at room temperature. Once resuspended, store frozen aliquots. Minimize freeze thaw cycles as much as possible. Freeze thaw will lower DNase activity.

Q3: Is the DNase-I treatment necessary?

If the downstream application requires DNA-free RNA, we recommend performing the DNase I treatment.

Q4: Is the kit compatible with samples stored in DNA/RNA Shield?

Yes, bring samples homogenized and stored in DNA/RNA Shield to room temperature (20-30ºC). Add 3 volume of TRIzol/TRI Reagent and mix well. Proceed with RNA Purification.

Q5: Is Direct-zol suitable for very small numbers of cells?

Yes, the Direct-zol MicroPrep (#R2060) is designed and capable of purifying RNA down to single cell inputs (picogram amounts). A sensitive quantification method is needed (e.g. Qubit, qPCR, etc.)

Q6: Is it possible to extract proteins with the Direct-zol RNA kits?

Yes, proteins can be Acetone Precipitated post RNA binding step. Please request supplementary protocol from Zymo Research Technical Support.

Q7: Can samples be stored in TRIzol/TRI Reagent prior to processing?

Yes, samples in TRIzol/TRI Reagent or similar are stable overnight at room temperature and can be stored frozen (-80C). Be sure to lyse and homogenize the sample well prior to freezing. Bring the sample to room temperature prior to RNA Purification.

Q8: Is it possible to isolate DNA with the Direct-zol RNA kits?

Direct-zol DNA/RNA (D2080) kits can isolate DNA from TRIzol.

Q9: Is the RNA suitable for Next-Gen sequencing or other sensitive downstream applications?

Yes, the RNA is high quality (A260/A280 >1.8, A260/A230 >1.8) and suitable for any downstream application, including NGS, RT-PCR, hybridization, etc.

Q10: Which phenol-based reagents are compatible with Direct-zol?

The Direct-zol kits are compatible with TRI Reagent, TRIzol, Qiazol, RNAzol, TriPure, TriSure, etc., and any other acid-guanidinium phenol-based reagents.

Q11: What is the difference between the Direct-zol RNA and Quick-RNA kits?

Direct-zol is for samples stored/collected into TRIzol/similar reagents. Quick-RNA is for all other samples.

Q12: What is the difference between the Direct-zol RNA MiniPrep and the Direct-zol RNA MiniPrep Plus?

Both kits function the same, the only difference is the RNA binding capacity of the column provided with the kit.

Q13: I ran out of RNA Wash Buffer. Can I use something else?

Yes, use 80% ethanol as a substitute. RNA Wash Buffer is also sold separately.




“No phase separation was needed, but you still had the benefits of a Trizol extraction. No need to precipitate and resuspend samples, which means less sample loss during purification.”

-Adina B. (University of Guelph)

“This kit is amazing, I"ve got a gel comparing the lack of gDNA as shown in the advertising pamphlet. What can I say, except: I love this product!“

-R.K. CSU

“Direct-zol is the most excellent kit for RNA isolation that I ever used in the past 20 years.”

-H.Z. (Harvard Medical School)
Read More

Cat #NameSizePrice
E1010-1-16DNA Digestion Buffer16 mL$29.00
R2050-1-200TRI Reagent200 ml$219.00
E1010-1-4DNA Digestion Buffer4 mL$15.00
C2003Elution Plate2 Plates$19.00
C2002Collection Plate2 Plates$22.00
C2007-296-Well Plate Cover Foil2 Foils$10.00
C2007-496-Well Plate Cover Foil4 Foils$10.00
C2004Zymo-Spin I-96 Plate2 Plates$142.00
W1001-30DNase/RNase-Free Water30 ml$22.00
W1001-10DNase/RNase-Free Water10 ml$19.00
R2050-2-160Direct-zol RNA PreWash (Concentrate)160 ml$166.00
R2054Direct-zol-96 RNA2 x 96 preps$418.00
R2056Direct-zol-96 RNA4 x 96 preps$675.00
R1003-3-48RNA Wash Buffer48 ml$105.00
E1010DNase I Set250 U$56.00
品牌介绍
ZYMO RESEARCH生物科技公司位于美国加州Orange County,创建于上世纪90年代初,主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出,独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络,销售额快速增长。使用其产品的实验论文在各种权威性科技期刊(NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等)上发表。