4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
主营:主营:分子类,蛋白类,抗体类,生化类试剂
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > ZYMO RESEARCH/Quick DNA Plant/Seed Miniprep试剂盒/D6020
商品详细ZYMO RESEARCH/Quick DNA Plant/Seed Miniprep试剂盒/D6020
ZYMO RESEARCH/Quick DNA Plant/Seed Miniprep试剂盒/D6020
ZYMO RESEARCH/Quick DNA Plant/Seed Miniprep试剂盒/D6020
商品编号: D6020
品牌: zymoresearch
市场价: ¥4320.00
美元价: 2592.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Inhibitor-free DNA isolated directly from plant and seed samples.
Highlights

  • Rapid and simple method for DNA isolation from tough-to-lyse plant and seed samples.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin column technology coupled with filtration removes polyphenolic PCR inhibitors from the DNA product.
Description

The Quick-DNA Plant/Seed Miniprep Kit is a plant DNA isolation kit designed for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, seeds, etc. The procedure is easy and can be completed in minutes: plant samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Polysaccharides, lipids, and polyphenols/tannins are removed from the DNA using our Zymo-Spin column technology. The eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, etc.


Applicable ForAll sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume≥ 50 µl
EquipmentMicrocentrifuge, vortex, cell disruptor/pulverizer
Processing Volume≤ 150 mg
PurityA260/A280 nm ≥1.8.
Sample SourceLeaves, stems, buds, flowers, fruit, seeds, etc.
Sample StorageDNA stored at ≤ -20°C.
Size RangeCapable of recovering genomic DNA up to and above 40 kb. Typical fragment sizes range from 25 kb - 35 kb. If present, parasitic and viral DNA will also be recovered.
TypeTotal DNA
Yield≤ 25 µg total DNA

Q1: Can you provide a list of the tested plant species?

We currently do not have a list of plants, aside from what we have shown in the protocols:–A.thaliana–Juniper–Milkweed Leaf–Milkweed Leaflet–Milkweed Pre-Flowering Bud–Corn Kernel–Sunflower Seed–Nicotiana sp.

Q2: My lysate seems viscous. What is causing this to happen? How can I fix this?

A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

Q3: Are there any tips in optimzing bead beating conditions?

We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 2 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.

Q4: What is the purpose of Zymo-Spin III-HRC step?

Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that often co-purify and affect downstream applications such as PCR. The Zymo-Spin III-HRC column removes these polyphenolic PCR inhibitors to recover DNA that is ready for sensitive downstream applications such as Next-Gen Sequencing, quantitative PCR, etc.

Q5: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

Q6: When can an RNase A treatment be implemented in the protocol?

The Quick-DNA kits recover RNA-free genomic DNA. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through. No RNase A treatment is required when processing samples within kit capacity.



Cat #NameSizePrice
D3004-1-100Genomic Lysis Buffer100 ml$60.00
D3004-2-50g-DNA Wash Buffer50 ml$18.00
D3004-4-10DNA Elution Buffer10 ml$14.00
D3004-5-250DNA Pre-Wash Buffer250 ml$71.00
D6001-3-40BashingBead Buffer40 ml$29.00
D6035-1-30Prep Solution30 ml$18.00
C1057-50Zymo-Spin III-F Filters50 Pack$59.00
C1058-50Zymo-Spin III-HRC Filters50 Pack$108.00
S6003-50ZR BashingBead Lysis Tubes (2 mm)50 Tubes$101.00
C1078-50Zymo-Spin IICR Columns50 Pack$55.00
品牌介绍
ZYMO RESEARCH生物科技公司位于美国加州Orange County,创建于上世纪90年代初,主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出,独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络,销售额快速增长。使用其产品的实验论文在各种权威性科技期刊(NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等)上发表。