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当前位置: 首页 > 产品中心 > Small_molecule > ZYMO RESEARCH/Quick-DNA Fecal/Soil Microbe 96 Kit/D6011
商品详细ZYMO RESEARCH/Quick-DNA Fecal/Soil Microbe 96 Kit/D6011
ZYMO RESEARCH/Quick-DNA Fecal/Soil Microbe 96 Kit/D6011
ZYMO RESEARCH/Quick-DNA Fecal/Soil Microbe 96 Kit/D6011
商品编号: D6011
品牌: zymoresearch
市场价: ¥13460.00
美元价: 8076.00
产地: 美国(厂家直采)
公司:
产品分类: 小分子
公司分类: Small_molecule
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Extract high quality, inhibitor-free metagenomic DNA from feces, soil, water, etc.
Highlights

  • Rapid, high-throughput (96-well) method for the isolation of inhibitor-free, PCR-quality DNA from fecal samples in minutes, including those from humans, birds, rats, mice, cattle, etc.
  • Ultra-high density BashingBeads are fracture resistant and chemically inert.
  • Zymo-Spin plate unique filtration technologies effectively removes PCR inhibitors from the DNA product.
Description

The Quick-DNA Fecal/Soil Microbe 96 Kit is a fecal and soil DNA extraction kit designed for the simple and rapid isolation of inhibitor-free, PCR-quality host cell and microbial DNA from a variety of sample sources, including humans, birds, rats, mice, cattle, etc. The procedure is easy and can be completed in minutes: fecal samples are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads. Zymo-Spin technology is then used to isolate the DNA which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. Eluted DNA is ideal for downstream molecular-based applications including PCR, arrays, genotyping, methylation detection, etc.


Applicable ForAll sensitive downstream applications such as qPCR and Next-Generation Sequencing.
Elution Volume≥ 50 µl
EquipmentCentrifuge w/ microplate carriers, 96-well plate/block disruptor or pulverizer.
Processing Volume≤80mg feces, ≤ 135 mg soil, ≤20 mg fungal/bacterial cells (wet weight)
PurityA260/A280 nm ≥1.8.
Sample SourceFeces or soil
Sample StorageDNA stored at ≤ -20°C.
Size RangeCapable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered.
TypeTotal DNA
Yield≤ 5 µg total DNA

Q1: My lysate seems viscous. What is causing this to happen? How can I fix this?

A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

Q2: What is the purpose of Silicon-A-HRC Plate step?

Environmental samples often contain inhibitors such as polyphenolics, humic/fulvic acids, tannins, melanins, etc. that affect downstream applications such as PCR. Once the DNA is eluted off the binding spin plate, the DNA is then passed through the Silicon-A-HRC Plate to remove the PCR inhibitors, and the DNA is then ready for downstream applications. The Silicon-A-HRC Plate does not bind DNA, it simply removes the PCR inhibitors. The Silicon-A-HRC Plate can be purchased separately as the OneStep-96 PCR Inhibitor Kit (D6035).

Q3: Is it necessary to add beta-mercaptoethanol? Can this step be substituted or omitted?

Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

Q4: When can an RNase A treatment be implemented in the protocol?

No additional RNase A treatment is required when processing samples within kit capacity. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through.



Cat #NameSizePrice
S6002-96-3ZR-96 BashingBead Lysis Rack0.5 mm & 0.1 mm$192.00
C2009Silicon-A-HRC Plate2 Plates$405.00
C2003Elution Plate2 Plates$19.00
C2002Collection Plate2 Plates$22.00
C2007-496-Well Plate Cover Foil4 Foils$10.00
P1001-296-Well Block2 Blocks$18.00
C2001Silicon-A Plate2 Plates$129.00
D6035-1-30Prep Solution30 ml$18.00
D6001-3-40BashingBead Buffer40 ml$29.00
D3004-5-50DNA Pre-Wash Buffer50 ml$26.00
D3004-4-10DNA Elution Buffer10 ml$14.00
D3004-2-100g-DNA Wash Buffer100 ml$30.00
D3004-1-150Genomic Lysis Buffer150 ml$73.00
品牌介绍
ZYMO RESEARCH生物科技公司位于美国加州Orange County,创建于上世纪90年代初,主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出,独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络,销售额快速增长。使用其产品的实验论文在各种权威性科技期刊(NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等)上发表。