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当前位置: 首页 > 产品中心 > Two_Hybrid_Systems > ZYMO RESEARCH/Quick DNA 96 Plus试剂盒/4 x 96 Preps/D4070
商品详细ZYMO RESEARCH/Quick DNA 96 Plus试剂盒/4 x 96 Preps/D4070
ZYMO RESEARCH/Quick DNA 96 Plus试剂盒/4 x 96 Preps/D4070
ZYMO RESEARCH/Quick DNA 96 Plus试剂盒/4 x 96 Preps/D4070
商品编号: D4070
品牌: zymoresearch
市场价: ¥19780.00
美元价: 11868.00
产地: 美国(厂家直采)
公司:
产品分类: 双杂交系统
公司分类: Two_Hybrid_Systems
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Streamlined DNA isolation from any cells, tissues, and biological fluids (Proteinase K included).
Highlights

  • Extract high-quality DNA easily and reliably from any biological fluids, cultured/monolayer cells, or solid tissues.
  • Zymo-Spin Technology ensures DNA is ready for all sensitive downstream applications such as qPCR, DNA-sequencing, arrays, and methylation analysis.
Description

The Quick-DNA 96 Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 10 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free bypassing the need for RNase A treatment and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.


Elution Volume≤ 15 µl
EquipmentWater bath or heat block (55°C), microcentrifuge, and vortex.
PurityHigh quality DNA is ready for all sensitive downstream applications such as PCR, endonuclease digestion, Southern blotting, genotyping, Next-Gen sequencing, bisulfite conversion, etc. (A260/A230≥ 2.0).
Size RangeCapable of recovering genomic and mitochondrial DNA sized fragments > 50 kb. If present, parasitic, microbial, and viral DNA will also be recovered.
WorkflowUtilize a Proteinase K Digestion and Zymo-Spin Column for effective recovery of DNA.
YieldThe DNA binding capacity of the well is 5 µg. Typically, mammalian tissues yield: 1-3 µg DNA per mg skeletal, heart, lung, and brain tissues and 3-5 µg DNA per mg liver and kidney. Human whole blood will yield 3-7 µg DNA per 100 µl blood sampled.

Q1: Can I use Quick-DNA Plus to clean-up previously isolated DNA?

No, the kit is designed for direct use with biological samples. For clean-up of previously isolated DNA, please use the Genomic DNA Clean & Concentrator or the DNA Clean & Concentrator kits.

Q2: What are the expected yields for each sample type?

Keep in mind that there is sample to sample variability.

Sample TypeInput Amount Expected Yield
Eukaryotic Cells1x106 Cells 5-6 µg
Skeletal, Heart, Lung, Brain Tissue1 mg 1-3 µg
Liver and Kidney Tissue1 mg 3-5 µg
Human Whole Blood100 µl 5-7 µg

Q3: I’m seeing some yield inconsistencies with my blood samples, what’s happening?

White blood cells, which are the major source of genomic DNA in blood, easily and quickly settles. Mix the blood sample well prior to aliquoting for purification.

Q4: Can Proteinase K digestion be performed overnight in DNA/RNA Shield?

Yes, samples can be digested overnight. Make sure to follow the appendix (page 8 appendix B) for processing liquids samples in DNA/RNA Shield and incubate at room temperature.

Q5: What is the difference between Quick-DNA and Quick-DNA Plus kits?

The Quick-DNA is optimized for cells, soft tissues, and homogenized/digested samples using a single lysis/binding buffer. The Quick-DNA Plus kits contain an optimized Proteinase K for processing a wider variety of sample inputs, such as cells, blood, tissues, etc. The upgraded Quick-DNA Plus recovers more DNA with higher purity compared to the Quick-DNA Kits.

Q6: Can the Quick-DNA Plus kit be used with bacterial samples?

E.coli cells are easy-to-lyse and can be processed directly with the Biological Fluids & Cells protocol. For an all-inclusive kit with any type of microbes (including tough-to-lyse), use any of Zymo Research’s Environmental Kits (e.g. Quick-DNA Fungal/Bacterial, Quick-DNA Fecal/Soil, ZymoBIOMICS DNA, etc.).

Q7: What is the difference between the digestion buffers? Can one buffer be used for all sample types?

The BioFluid & Cell Buffer (Red) is designed to allow for rapid Proteinase K digestion with easy-to-lyse samples (e.g. mammalian cells and biological fluids).The Solid Tissue buffer (Blue) can be used for any sample type and requires a longer PK digestion time compared to using the BioFluid & Cell Buffer (Red).

Q8: Can Quick-DNA process crude lysates?

Yes, add 4 volumes of Genomic Lysis Buffer to 1 volume of crude lysate, homogenized, or digested sample (see Cell Suspensions and Proteinase K Digested Samples) and proceed with the remainder of the protocol.

Q9: What is the purpose of adding beta-mercaptoethanol? Can this step be substituted or omitted?

Beta-mercaptoethanol is a reducing agent that helps break down proteins and improves DNA recovery and purity. Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM) or omitted.



Cat #NameSizePrice
P1002-296-Well Block w/ Cover Foil2 Blocks/foils$28.00
C2010Zymo-Spin I-96-XL Plates2 Plates$257.00
C2007-496-Well Plate Cover Foil4 Foils$10.00
C2007-296-Well Plate Cover Foil2 Foils$10.00
C2003Elution Plate2 Plates$19.00
C2002Collection Plate2 Plates$22.00
D4068-3-85Genomic Binding Buffer85 ml$95.00
D4068-2-10Solid Tissue Buffer (Blue)10 ml$24.00
D4068-3-45Genomic Binding Buffer45 ml$49.00
D4068-2-22Solid Tissue Buffer (Blue)22 ml$38.00
D4068-1-12BioFluid & Cell Buffer (Red)12 ml$24.00
D4068-1-25BioFluid & Cell Buffer (Red)25 ml$38.00
D3004-5-50DNA Pre-Wash Buffer50 ml$26.00
D3004-4-10DNA Elution Buffer10 ml$14.00
D3004-2-50g-DNA Wash Buffer50 ml$18.00
D3004-2-100g-DNA Wash Buffer100 ml$30.00
品牌介绍
ZYMO RESEARCH生物科技公司位于美国加州Orange County,创建于上世纪90年代初,主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出,独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络,销售额快速增长。使用其产品的实验论文在各种权威性科技期刊(NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等)上发表。