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当前位置: 首页 > 产品中心 > CRISPR > 酵母菌研究/基因组DNA清洁浓缩器-25/100 Preps/D4064
商品详细酵母菌研究/基因组DNA清洁浓缩器-25/100 Preps/D4064
酵母菌研究/基因组DNA清洁浓缩器-25/100 Preps/D4064
酵母菌研究/基因组DNA清洁浓缩器-25/100 Preps/D4064
商品编号: D4064
品牌: zymoresearch
市场价: ¥5960.00
美元价: 3576.00
产地: 美国(厂家直采)
公司:
产品分类: CRISPR基因敲除
公司分类: CRISPR
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Genomic DNA Clean & Concentrator-25 (DCC) 25
Highlights

  • Quick (5 minute) clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations
  • Unique spin column for low volume elution of ultra-pure, high-yield DNA
  • Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
Description

The Genomic DNA Clean & Concentrator-25 (DCC) is a spin column gDNA clean up kit that provides quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga) DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.


Applicable ForEluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Elution Volume≥ 35 µl of DNA Elution Buffer
EquipmentMicrocentrifuge
PurityA260/A280 > 1.8, A260/A230 > 1.8
Sample SourceEnzymatic reactions or impure preparations containing genomic DNAs
Sample storageEluted DNA can be used immediately or stored at ≤ -20°C
Size Range50 bp to 200 kb
YieldRecovery of DNA ranges from 70 - 95%

Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q2: How to process naked DNA stored in DNA/RNA Shield?

Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).

Q3: What to do if ethanol addition to the DNA Wash Buffer was omitted?

The DNA will be eluted off the column. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How many times can columns be reloaded?

We recommend no more than 5 times as binding efficiency might decrease.

Q6: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. We recommend raising the starting volume to 100 µl with water to ensure optimal binding conditions.



Cat #NameSizePrice
C1102-25Zymo-Spin IIC-XL Columns25 Pack$34.00
D5201-1-50ChIP DNA Binding Buffer50 ml$37.00
C1001-1000Collection Tubes1000 Pack$90.00
C1001-500Collection Tubes500 Pack$52.00
C1001-50Collection Tubes50 Pack$15.00
D4003-2-6DNA Wash Buffer (Concentrate)6 ml$10.00
D4003-2-24DNA Wash Buffer (Concentrate)24 ml$33.00
D3004-4-10DNA Elution Buffer10 ml$14.00
D3004-4-1DNA Elution Buffer1 ml$11.00
D3004-4-4DNA Elution Buffer4 ml$10.00
品牌介绍
ZYMO RESEARCH生物科技公司位于美国加州Orange County,创建于上世纪90年代初,主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出,独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络,销售额快速增长。使用其产品的实验论文在各种权威性科技期刊(NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等)上发表。