酵母菌研究/基因组DNA清洁浓缩器-10/25 Preps/D4010-免疫在线蚂蚁淘旗下平台

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商品详细酵母菌研究/基因组DNA清洁浓缩器-10/25 Preps/D4010

酵母菌研究/基因组DNA清洁浓缩器-10/25 Preps/D4010

商品编号： D4010

品牌： zymoresearch

市场价： ¥1820.00

美元价： 1092.00

产地：美国(厂家直采)

公司：

产品分类：克隆基因

公司分类： Cloned_Genes

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Purify high molecular weight DNA from enzymatic reactions and dilute samples.

Highlights

Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
Unique spin column for low volume elution of ultra-pure, high-yield DNA.
Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.

Description

The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.

Applicable For	Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application
Elution Volume	≥ 10 µl of DNA Elution Buffer
Equipment	Microcentrifuge
Purity	A260/A280 > 1.8, A260/A230 > 1.8
Sample Source	Enzymatic reactions or impure preparations containing genomic DNA.
Sample Storage	Eluted DNA can be used immediately or stored at ≤ -20°C.
Size Range	50 bp to 200 kb
Yield	Recovery of DNA ranges from 70 - 95%

Q1: What is the lower limit and minimal amount of DNA that can be recovered?

Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.

Q2: How can I process naked DNA stored in DNA/RNA Shield?

Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).

Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?

The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.

Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?

Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.

Q5: How many times can columns be reloaded?

Zymo Research recommends no more than 5 times, as binding efficiency might decrease.

Q6: What is the minimum input volume of DNA sample?

Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions.

Cat #	Name	Size	Price
C1002-25	Zymo-Spin IC-XL	25 Pack	$34.00
C1002-50	Zymo-Spin IC-XL	50 Pack	$66.00
D5201-1-50	ChIP DNA Binding Buffer	50 ml	$37.00
C1001-50	Collection Tubes	50 Pack	$15.00
C1001-500	Collection Tubes	500 Pack	$52.00
C1001-1000	Collection Tubes	1000 Pack	$90.00
D4003-2-6	DNA Wash Buffer (Concentrate)	6 ml	$10.00
D4003-2-24	DNA Wash Buffer (Concentrate)	24 ml	$33.00
D3004-4-1	DNA Elution Buffer	1 ml	$11.00
D3004-4-4	DNA Elution Buffer	4 ml	$10.00

品牌介绍

ZYMO RESEARCH生物科技公司位于美国加州Orange County，创建于上世纪90年代初，主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出，独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络，销售额快速增长。使用其产品的实验论文在各种权威性科技期刊（NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等）上发表。

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