ZYMO研究/基因组DNA清洁浓缩器-10/100 Preps/D4011
商品编号：
D4011
品牌：
zymoresearch
市场价：
¥5960.00
美元价：
3576.00
产品分类：
RNA干扰
公司分类：
RNA_interference
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Highlights
- Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
- Unique spin column for low volume elution of ultra-pure, high-yield DNA.
- Eluted DNA is ideal for PCR, endonuclease digestion, sequencing, etc.
Description
| Applicable For | Eluted DNA is ideal for ligation, sequencing, labeling, PCR, microarray, transfection, transformation, restriction digestion procedures, and any other sensitive downstream application |
|---|---|
| Elution Volume | ≥ 10 µl of DNA Elution Buffer |
| Equipment | Microcentrifuge |
| Purity | A260/A280 > 1.8, A260/A230 > 1.8 |
| Sample Source | Enzymatic reactions or impure preparations containing genomic DNA. |
| Sample Storage | Eluted DNA can be used immediately or stored at ≤ -20°C. |
| Size Range | 50 bp to 200 kb |
| Yield | Recovery of DNA ranges from 70 - 95% |
Q1: What is the lower limit and minimal amount of DNA that can be recovered?
Picogram levels of DNA can be recovered. The limitation is based on sensitivity of detection method.
Q2: How can I process naked DNA stored in DNA/RNA Shield?
Use the standard protocol (add 2 or 5 volumes of DNA binding buffer depending on DNA size).
Q3: What should I do if I did not add ethanol to the DNA Wash Buffer before starting?
The DNA will be eluted off the column during the wash step. Rebind samples using the appropriate amount of DNA Binding Buffer and wash the column with the properly prepared wash buffer.
Q4: What happens if more DNA was loaded on the columns than the stated maximum binding capacity?
Oversaturation of the column can result in total DNA loss due to clogging of silica matrix.
Q5: How many times can columns be reloaded?
Zymo Research recommends no more than 5 times, as binding efficiency might decrease.
Q6: What is the minimum input volume of DNA sample?
Working with volumes below 50 µl can result in decreased recovery. Zymo Research recommends raising the starting volume to 100 µl with water to ensure optimal binding conditions.
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1002-25 | Zymo-Spin IC-XL | 25 Pack | $34.00 | |
| C1002-50 | Zymo-Spin IC-XL | 50 Pack | $66.00 | |
| D5201-1-50 | ChIP DNA Binding Buffer | 50 ml | $37.00 | |
| C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
| C1001-500 | Collection Tubes | 500 Pack | $52.00 | |
| C1001-1000 | Collection Tubes | 1000 Pack | $90.00 | |
| D4003-2-6 | DNA Wash Buffer (Concentrate) | 6 ml | $10.00 | |
| D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
| D3004-4-1 | DNA Elution Buffer | 1 ml | $11.00 | |
| D3004-4-4 | DNA Elution Buffer | 4 ml | $10.00 |
品牌介绍
ZYMO RESEARCH生物科技公司位于美国加州Orange County，创建于上世纪90年代初，主要研发和生产生物科技实验室使用的各种生物试剂、耗材、仪器。其DNA提取试剂盒系列产品以“快速、简便、超纯”的特点在众多生物科技产品中脱颖而出，独占鳌头。已经形成了覆盖美、英、德、法、日、韩等50多个国家的销售网络，销售额快速增长。使用其产品的实验论文在各种权威性科技期刊（NATURE 自然、PNAS 美国科学院院刊、StemCells 干细胞、Nucleic Acids Res核酸研究等）上发表。
品牌分类
唾液收集的
\基因组DNA干净
\Zymoclean DNA凝胶回收试剂盒\
组织收集的
Quick-DNA Plant/Seed Kits
粪便/土壤微生物快速DNA试剂盒
\DNA干净
\除OneStep PCR抑制剂包\
\ < \/ i >快速dna粪便\/土壤微生物板\
快速DNA试剂盒
XJ Autolysis E. coli strains
\ < \/ i > dna快速包\
混合
\向量集合和环境\
\Zymoprep酵母质粒Miniprep包\
Quick-DNA Fungal/Bacterial Kits
\ < \/ i >快速dna植物\/种子包
\粪便收集\
混合和去活性细胞
\XJ自我分解 E。"},{"src":"coli strains","tgt":"杆菌< \/ i >菌株的
血液采集的
\Select-a-Size DNA干净
尿液收集的
\ < \/ i >快速dna真菌\/细菌板\
\Direct-zol RNA包\
显示分类
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